Journal: Journal of Cosmetic Dermatology

Article Title: Synergistic Anti‐Aging Effects of Adipose‐Derived Stem Cell Extracellular Vesicles Loaded With Natural Compounds

doi: 10.1111/jocd.70021

Figure Lengend Snippet: Screening for the highest combined anti‐oxidant activity. (A) Experimental design of the screen for cytotoxicity and anti‐oxidant effect using human keratinocyte HaCaT cells. ROS measurement was performed in HaCaT cells irradiated with UVB and treated with different concentrations of (B) human ASC‐sEVs, (C) selected natural compounds, and (D) human ASC‐sEVs loaded with compounds. * p < 0.05, student's t ‐test. NR, nicotinamide riboside; RES, resveratrol; ROS, reactive oxygen species; UVB, ultraviolet B; VITC, vitamin C.

Article Snippet: The cells were then incubated with different sEV preparations or compounds for 48 h. For ROS measurement, cells were first stained with 25 μM DCFDA (ab113851, Abcam) for 45 min in the dark [ ], then washed with PBS and exposed under UVB Narrowband lamps (PL‐S 9 W, Philips) at 30 mJ/cm 2 .

Techniques: Activity Assay, Irradiation

Journal: Journal of Cosmetic Dermatology

Article Title: Synergistic Anti‐Aging Effects of Adipose‐Derived Stem Cell Extracellular Vesicles Loaded With Natural Compounds

doi: 10.1111/jocd.70021

Figure Lengend Snippet: Screening for the highest combined anti‐wrinkle activity. (A) Experimental design of the screen for cytotoxicity and anti‐wrinkle effect using human keratinocyte HaCaT cells. (B) The morphology of HaCaT cells with or without UVB irradiation. Gene expressions of MMP1 , MMP3 , and PLOD1 were measured in HaCaT cells irradiated with UVB and treated with different concentrations of (C) human ASC‐sEVs, (D) selected natural compounds, and (E) human ASC‐sEVs loaded with compounds. * p < 0.05, student's t ‐test. MMP, matrix metalloproteinase; PLOD1, procollagen‐lysine 2‐oxoglutarate 5‐dioxygenase 1; RES, resveratrol; RET, retinol; UVB, ultraviolet B; VITC, vitamin C.

Article Snippet: The cells were then incubated with different sEV preparations or compounds for 48 h. For ROS measurement, cells were first stained with 25 μM DCFDA (ab113851, Abcam) for 45 min in the dark [ ], then washed with PBS and exposed under UVB Narrowband lamps (PL‐S 9 W, Philips) at 30 mJ/cm 2 .

Techniques: Activity Assay, Irradiation

Journal: Journal of Cosmetic Dermatology

Article Title: Synergistic Anti‐Aging Effects of Adipose‐Derived Stem Cell Extracellular Vesicles Loaded With Natural Compounds

doi: 10.1111/jocd.70021

Figure Lengend Snippet: Screening for the highest combined anti‐melanogenic activity. (A) Experimental design of the screen for cytotoxicity and anti‐melanogenic effect using human melanocyte B16F10 cells. Intracellular and extracellular melanin levels were measured from B16F10 cell culture treated with different concentrations of (B) human ASC‐sEVs, (C) selected natural compounds, and (D) human ASC‐sEVs loaded with compounds. * p < 0.05, student's t ‐test. ARB, arbutin; NR, nicotinamide riboside; RES, resveratrol; RET, retinol; UVB, ultraviolet B; VITC, vitamin C.

Article Snippet: The cells were then incubated with different sEV preparations or compounds for 48 h. For ROS measurement, cells were first stained with 25 μM DCFDA (ab113851, Abcam) for 45 min in the dark [ ], then washed with PBS and exposed under UVB Narrowband lamps (PL‐S 9 W, Philips) at 30 mJ/cm 2 .

Techniques: Activity Assay, Cell Culture

Journal: Cell Death & Disease

Article Title: ZBP1-mediated PANoptosis is a crucial lethal form in diverse keratinocyte death modalities in UVB-induced skin injury

doi: 10.1038/s41419-025-07351-3

Figure Lengend Snippet: A Photos of keratinocyte-specific Gsdmd cKO mice (Krt14 Cre/+ - Gsdmd flox/flox ) and control mice (Krt14 +/+ - Gsdmd flox/flox ) at 48 h after exposure or non-exposure to 430 mJ/cm 2 UVB irradiation (n = 4). B UVB skin damage of Krt14 Cre/+ - Gsdmd flox/flox mice and Krt14 +/+ - Gsdmd flox/flox mice was evaluated by scores (n = 4). C Representative images of H&E, TUNEL, MPO, MMP9, F4/80 staining in Krt14 Cre/+ - Gsdmd flox/flox mice and Krt14 +/+ - Gsdmd flox/flox mice after exposure or non-exposure to UVB irradiation. The statistic of epidermal thickness and average optical densities of TUNEL, MPO, MMP9, F4/80 positive staining were analyzed (n = 3). D Proteins of N-GSDMD, cleaved PARP, cleaved caspase-3, and p-MLKL in the epidermis of Krt14 Cre/+ - Gsdmd flox/flox mice and Krt14 +/+ - Gsdmd flox/flox mice were detected by western blotting assay. The relative protein levels of the interested proteins were compared with ACTB as quantification (n = 3). E Flowchart of DSF treatment or vehicle treatment for acute UVB skin injury model in WT mice (48 h after 430 mJ/cm 2 UVB irradiation). F Representative photos of mice receiving DSF or vehicle treatment at 48 h after UVB exposure. G UVB skin damage of DSF or vehicle treated mice was evaluated by scores (n = 8). H Representative images of H&E, TUNEL, MPO, MMP9, and F4/80 staining in DSF or vehicle treated mice after UVB irradiation. Statistical analysis of epidermal thickness and average optical densities of TUNEL, MPO, MMP9, F4/80 positive staining were shown (n = 8). I Proteins of GSDMD-FL, N-GSDMD, cleaved PARP, cleaved caspase-3, and p-MLKL in the epidermis of DSF or vehicle treated mice were detected by western blotting assay. The relative protein levels of the interested proteins were compared with ACTB as quantification (n = 3). The scale bar in black is 100 μm for 10× magnification in C and H . *P < 0.05. **P < 0.01. ***P < 0.001. ns not significant. DSF disulfiram. GSDMD-FL GSDMD full length. N-GSDMD N-terminal GSDMD. p-MLKL phosphorylated MLKL.

Article Snippet: Human primary keratinocytes were exposed to 50 mJ/cm 2 UVB by UVB lamps (Philips, UVB Broadband PL-S 9 W/12) and collected after 12 h of UVB irradiation.

Techniques: Control, Irradiation, TUNEL Assay, Staining, Western Blot

Journal: Cell Death & Disease

Article Title: ZBP1-mediated PANoptosis is a crucial lethal form in diverse keratinocyte death modalities in UVB-induced skin injury

doi: 10.1038/s41419-025-07351-3

Figure Lengend Snippet: A Photos of keratinocyte-specific Mlkl cKO mice (Krt14 Cre/+ - Mlkl flox/flox ) and control mice (Krt14 +/+ - Mlkl flox/flox ) at 48 h after exposure or non-exposure to 430 mJ/cm 2 UVB irradiation (n = 5). B UVB skin damage of Krt14 Cre/+ - Mlkl flox/flox mice and Krt14 +/+ - Mlkl flox/flox mice was evaluated by scores (n = 5). C Representative images of H&E, TUNEL, MPO, MMP9, and F4/80 staining in Krt14 Cre/+ - Mlkl flox/flox mice and Krt14 +/+ - Mlkl flox/flox mice after exposure or non-exposure to UVB irradiation. Statistical analysis of epidermal thickness and average optical densities of TUNEL, MPO, MMP9, F4/80 positive staining were shown (n = 5). D Proteins of N-GSDMD, cleaved PARP, cleaved caspase-3, MLKL and p-MLKL in the epidermis of Krt14 Cre/+ - Mlkl flox/flox mice and Krt14 +/+ - Mlkl flox/flox mice were detected by western blotting assay. The relative protein levels of the interested proteins were compared with ACTB as quantification (n = 3). The scale bar is 100 μm in C . *P < 0.05. **P < 0.01. ***P < 0.001. ns not significant. N-GSDMD N-terminal GSDMD. p-MLKL phosphorylated MLKL.

Article Snippet: Human primary keratinocytes were exposed to 50 mJ/cm 2 UVB by UVB lamps (Philips, UVB Broadband PL-S 9 W/12) and collected after 12 h of UVB irradiation.

Techniques: Control, Irradiation, TUNEL Assay, Staining, Western Blot

Journal: Cell Death & Disease

Article Title: ZBP1-mediated PANoptosis is a crucial lethal form in diverse keratinocyte death modalities in UVB-induced skin injury

doi: 10.1038/s41419-025-07351-3

Figure Lengend Snippet: A Constitutive proteins related to PANoptosome including ZBP1, AIM2, NLRP3, NLRP12, RIPK1, RIPK3, p-RIPK3, caspase-8, cleaved caspase-1, FADD, and ASC in mice epidermal lysates were detected by western blotting assay at 48 h after 430 mJ/cm 2 UVB irradiation. B Proteins of N-GSDMD, cleaved PARP, cleaved caspase-3, p-MLKL, and ZBP1 in human primary keratinocytes were detected by western blotting assay after UVB irradiation. C Representative images of immunohistochemical staining of ZBP1 in human skin samples from sun-exposed and non-exposed areas. Statistical analysis of average optical density of ZBP1 positive staining was shown (n = 12). D Representative photos of WT mice and Zbp1 −/− mice at 48 h after 430 mJ/cm 2 UVB exposure. E UVB skin damage of WT mice and Zbp1 −/− mice was evaluated by scores (n = 5). F Representative images of H&E staining and TUNEL staining of skin sections from WT mice and Zbp1 −/− mice after UVB irradiation. The statistic of epidermal thickness and average optical density of TUNEL positive staining were analyzed (n = 5). G Representative images of MPO, MMP9, and F4/80 immunohistochemical staining in WT mice and Zbp1 −/− mice after UVB irradiation. The statistic of average optical densities of MPO, MMP9, and F4/80 positive staining were analyzed (n = 5). H Proteins of ZBP1, N-GSDMD, cleaved PARP, cleaved caspase-3, and p-MLKL in the epidermis of WT mice and Zbp1 −/− mice were detected by western blotting assay. The relative protein levels of the above proteins were compared with ACTB as quantification (n = 3). The scale bar in black is 100 μm for 10× magnification in C , F , and G . The scale bar in yellow is 20 μm for 40× magnification in C and F . *P < 0.05. **P < 0.01. ns not significant. N-GSDMD N-terminal GSDMD. p-MLKL phosphorylated MLKL.

Article Snippet: Human primary keratinocytes were exposed to 50 mJ/cm 2 UVB by UVB lamps (Philips, UVB Broadband PL-S 9 W/12) and collected after 12 h of UVB irradiation.

Techniques: Western Blot, Irradiation, Immunohistochemical staining, Staining, TUNEL Assay